Recombinant Rat Cyt-C protein (GST tag)

Size: 100μg

Catalogue Numbers: PDER100036-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cyt-C

Target Synonym: CYC; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4

UNIProt ID: P62898

Research Areas: Cardiovascular; Cell Biology; Signal Transduction; Cancer; Metabolism

Target Species: Rat

Expression Host: E.coli

Fusion Tag: N-GST

Accession: P62898

Background: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

Sequence: Met 1-Glu 105

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 11.44 kDa

Observed MW: 28 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only